解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::Recently, accumulating studies have indicated that microRNAs (miRNAs) play an important role in exploring the pathogenesis of various human diseases at the molecular level and may result in the design of specific tools for diagnosis, treatment evaluation and prevention. Experimental identification of disease-related m...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00049e
更新日期:2016-06-21 00:00:00
abstract::The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00168h
更新日期:2016-06-01 00:00:00
abstract::Here, we used stopped-flow fluorescence techniques to conduct a comparative kinetic analysis of the conformational transitions in human apurinic/apyrimidinic endonuclease 1 (APE1) and in DNA containing an abasic site in the course of their interaction. Effects of monovalent (K(+)) and divalent (Mg(2+), Mn(2+), Ca(2+),...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00128a
更新日期:2016-05-26 00:00:00
abstract::In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00887e
更新日期:2016-05-24 00:00:00
abstract::In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00203j
更新日期:2016-05-24 00:00:00
abstract::Protein tyrosine phosphatases are crucial regulators of signal transduction and function as antagonists towards protein tyrosine kinases to control reversible tyrosine phosphorylation, thereby regulating fundamental physiological processes. Growing evidence has supported the notion that reversible oxidative inactivati...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00847f
更新日期:2016-05-24 00:00:00
abstract::Histone deacetylases (HDACs) contribute to regulation of gene expression by mediating higher-order chromatin structures. They assemble into large multiprotein complexes that regulate activity and specificity. We report the development of small molecule probes with class IIa and pan-HDAC activity that contain photoreac...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00109b
更新日期:2016-05-24 00:00:00
abstract::When exposed to low concentrations of toxic chemicals, bacteria modulate the expression of a number of cellular processes. Typically, these processes include those related to porin production, dismutases, and metabolic fluxes. In Escherichia coli (E. coli), the expression of these systems is largely controlled by thre...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00263c
更新日期:2016-05-24 00:00:00
abstract::Affinity probes are useful tools for determining molecular targets and elucidating mechanism of action for novel, bioactive compounds. In the case of covalent inhibitors, activity based probes are particularly valuable for ensuring acceptable selectivity margins. However, there is a variety of bioorthogonal chemistry ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00024j
更新日期:2016-05-01 00:00:00
abstract::Endogenous thymidylate synthases, isolated from tissues or cultured cells of the same specific origin, have been reported to show differing slow-binding inhibition patterns. These were reflected by biphasic or linear dependence of the inactivation rate on time and accompanied by differing inhibition parameters. Consid...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00026f
更新日期:2016-04-01 00:00:00
abstract::A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00751h
更新日期:2016-03-01 00:00:00
abstract::Chronic inflammation can lead to the development of cancers and resolution of inflammation is an ongoing challenge. Inflammation can result from dysregulation of the epigenome and a number of compounds that modify the epigenome are in clinical use. In this study the anti-inflammatory and anti-cancer effects of a quina...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00554j
更新日期:2016-03-01 00:00:00
abstract::Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00642b
更新日期:2016-03-01 00:00:00
abstract::The identification of genetic markers (e.g. genes, pathways and subnetworks) for cancer has been one of the most challenging research areas in recent years. A subset of these studies attempt to analyze genome-wide expression profiles to identify markers with high reliability and reusability across independent whole-tr...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00684h
更新日期:2016-03-01 00:00:00
abstract::Lamellipodin (Lpd) protein plays an important role in the formation of lamellipodial protrusion which is crucial in actin dynamics, cell polarity and motility. Lpd promotes actin polymerization with the help of members of the Ena/VASP family of actin regulators and tethering them to actin filaments. It is well documen...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00731c
更新日期:2016-03-01 00:00:00
abstract::The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00747j
更新日期:2016-02-01 00:00:00
abstract::Drug combinations have been widely applied to treat complex diseases, like cancer, HIV and cardiovascular diseases. One of the most important characteristics for drug combinations is the synergistic effects among different drugs, that is to say, the combination effects are larger than the sum of individual effects. Al...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00599j
更新日期:2016-02-01 00:00:00
abstract::MicroiRNAs are genome encoded small double stranded RNAs that regulate expression of homologous mRNAs. With approximately 2500 human miRNAs and each having hundreds of potential mRNA targets, miRNA based gene regulation is quite pervasive in both development and disease. While there are numerous studies investigating ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00415b
更新日期:2016-01-01 00:00:00
abstract::The dairy bacterium Lactococcus lactis has to master a complicated task. It must control its essentially linear glycolytic pathway in such a fashion that, when the substrate, glucose, runs out, it retains enough phosphoenolpyruvate and fructose-1,6-bisphosphate to be able to restart glycolysis as soon as new glucose b...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00726g
更新日期:2016-01-01 00:00:00
abstract::Classical Hodgkin lymphoma (cHL) is a malignancy with complex pathogenesis. The hallmark of the disease is the presence of large mononucleated Hodgkin and bi- or multinucleated Reed/Sternberg (H/RS) cells. The origin of HRS cells in cHL is controversial as these cells show the coexpression of markers of several lineag...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00654f
更新日期:2016-01-01 00:00:00
abstract::Integrative pharmacology has been used to identify the key active constituents (KACs) of Buchang Naoxintong capsules (BNCs), a traditional Chinese medical preparation; this approach involves the evaluation of the content profiles and drug-like properties of the BNC constituents and development of an ingredient-target ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00460h
更新日期:2016-01-01 00:00:00
abstract::Epithelial to mesenchymal transition (EMT) is a biological process that plays a crucial role in cancer metastasis. Although studies regarding the EMT mechanisms are usual in terms of gene expression and protein functions, little is known about the involvement of lipids in EMT. In this work, an untargeted lipidomic ana...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00413f
更新日期:2015-12-01 00:00:00
abstract::Unbalanced uptake of Omega 6/Omega 3 (ω-6/ω-3) ratios could increase chronic disease occurrences, such as inflammation, atherosclerosis, or tumor proliferation, and methylation methods for measuring the ruminal microbiome fatty acid (FA) composition/distribution play a vital role in discovering the contribution of foo...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00325c
更新日期:2015-11-01 00:00:00
abstract::Protein methylation catalyzed by methyltransferases carries many important biological functions. Methylation and their regulatory enzymes are involved in a variety of human disease states, raising the possibility that abnormally methylated proteins can be disease markers and methyltransferases are potential therapeuti...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c5mb00259a
更新日期:2015-10-01 00:00:00
abstract::Large combinatorial libraries of macrocyclic peptides are a useful source of bioactive compounds. However, peptides are not generally cell permeable, so there is great interest in the development of methods to create large libraries of modified peptides. In particular, N-alkylation of peptides is known to improve thei...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00308c
更新日期:2015-10-01 00:00:00
abstract::Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00268k
更新日期:2015-09-01 00:00:00
abstract::Genome-wide RNA interference screens have greatly facilitated the identification of essential host factors (EHFs) for viral infections, whose knockdown effects significantly influence virus replication but not host cell viability. However, little has been done to link EHFs with another important host factor type, i.e....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00309a
更新日期:2015-09-01 00:00:00
abstract::Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00214a
更新日期:2015-08-01 00:00:00
abstract::It is not surprising that a large number of diseases related to amyloid fibril depositions are formed in various organs. Therefore, it is necessary to understand the transformation of native proteins into amyloid fibrils in order to clarify which key elements of this process determine the pathway of protein misfolding...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00273g
更新日期:2015-08-01 00:00:00
abstract::The underlying reasons for the differences in the virulence of various types of Ebola virus (EBOV) remain unknown. Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of EBOV. The higher disorder of these pro...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00240k
更新日期:2015-08-01 00:00:00
abstract::Cell culture metabolomics has demonstrated significant advantages in cancer research. However, its applications have been impeded by some influencing factors such as culture media, which could significantly affect cellular metabolic profiles and lead to inaccuracy and unreliability of comparative metabolomic analysis ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00019j
更新日期:2015-07-01 00:00:00
abstract::The human corticotropin-releasing factor receptor type 1 (CRF1R) is a class B G-protein-coupled receptor (GPCR), which mediates the response to stress and has been considered as a drug target for depression and anxiety. Based on the CRF1R-antagonist crystal structure, we study the binding mechanism of two distinct ant...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00159e
更新日期:2015-07-01 00:00:00
abstract::Fabry disease (FD) is an X-linked lysosomal storage disorder caused by a deficiency of the lysosomal hydrolase α-galactosidase A (α-GalA) that leads to the intra-lysosomal accumulation of globotriaosylceramide (Gb3) in various organ systems. As a consequence, a multisystems disorder develops, culminating in stroke, pr...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00707g
更新日期:2015-06-01 00:00:00
abstract::The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00071h
更新日期:2015-06-01 00:00:00
abstract::Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome l...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00131e
更新日期:2015-05-01 00:00:00
abstract::Migraine is a common brain disorder characterized by recurrent attacks of severe headaches and other neurological symptoms. In one-third of patients headaches are accompanied by auras, which consist of transient visual and sensory disturbances, believed to be caused by cortical spreading depression (CSD). CSD is chara...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00049a
更新日期:2015-05-01 00:00:00
abstract::Normalization of high-throughput molecular expression profiles secures differential expression analysis between samples of different phenotypes or biological conditions, and facilitates comparison between experimental batches. While the same general principles apply to microRNA (miRNA) normalization, there is mounting...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00711e
更新日期:2015-05-01 00:00:00
abstract::Conversion of the predominantly α-helical cellular prion protein (PrP(C)) to the misfolded β-sheet enriched Scrapie form (PrP(Sc)) is a critical event in prion pathogenesis. However, the conformational triggers that lead to the isoform conversion (PrP(C) to PrP(Sc)) remain obscure, and conjectures about the role of un...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00689e
更新日期:2015-05-01 00:00:00
abstract::The identification of disulfide bonds provides critical information regarding the structure and function of a protein and is a key aspect in understanding signaling cascades in biological systems. Recent proteomic approaches using digestion enzymes have facilitated the characterization of disulfide-bonds and/or oxidiz...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00688g
更新日期:2015-04-01 00:00:00